Semen analysis: preparation, results and what happens next

Make sense of sperm count, movement and shape. Heracles explains how to prepare, read your semen report and understand the next step.

Peter Grice
Medically reviewed by
Peter Grice, Clinical Lead
Table of contents

A semen analysis measures semen volume and sperm count, movement and shape. It is the main laboratory test for a possible male fertility problem and helps a clinician decide what to do next.

Good preparation makes the result more useful. Follow your testing laboratory’s collection instructions and read the measures together; one number cannot determine whether you can conceive.

Clear explanations matter when you are reading a fertility result. Heracles co-founders Professor Tet Yap and Professor Suks Minhas bring specialist expertise in male fertility and reproductive health. Meet the Heracles clinical team or contact the Heracles team.

What does a semen analysis measure?

A standard diagnostic analysis may report:

  • semen volume: the amount of fluid collected
  • sperm concentration: the number of sperm in each millilitre
  • total sperm number: the estimated number of sperm in the complete ejaculate
  • total motility: the proportion of sperm that are moving
  • progressive motility: the proportion moving forwards
  • morphology: the proportion meeting strict criteria for normal shape
  • vitality: the proportion of live sperm, measured when clinically relevant
  • pH and appearance: features of the seminal fluid that may add context.

The report may also note round cells, white blood cells, clumping or whether the sample liquefied as expected. These observations need clinical interpretation; they do not automatically show infection or explain a delay in conception.

How to prepare for a semen analysis

UK laboratories commonly ask for two to seven days without ejaculation before the sample. Some specify a narrower window, such as three to five days. Use the exact interval given by your laboratory, and record it accurately.

Tell the referring clinician or laboratory about:

  • a fever or significant illness in the preceding months
  • prescription medicines, testosterone, supplements, anabolic steroids and recreational drugs
  • difficulty ejaculating or collecting by masturbation
  • any sample lost during collection
  • a previous vasectomy, pelvic surgery or suspected retrograde ejaculation.

Do not change a prescription medicine simply to prepare for the test. Ask the prescriber whether it should be continued.

How to collect the sample

Instructions vary, but a diagnostic fertility sample will usually be collected as follows:

  1. Use only the sterile container supplied or approved by the laboratory.
  2. Wash and dry your hands and genital area as directed.
  3. Produce the sample by masturbation directly into the container.
  4. Do not use saliva, standard lubricants or an ordinary condom. These can contaminate the sample or affect sperm. A laboratory may provide an approved sperm-safe lubricant or special non-spermicidal collection condom if needed.
  5. Collect the whole ejaculate. The first part often contains the highest sperm concentration, so tell the laboratory if any was missed.
  6. Close and label the container exactly as instructed, including the time of collection.
  7. Deliver it within the laboratory's stated time and temperature conditions. Many NHS laboratories require arrival within about one hour and advise keeping the sample close to body temperature without artificially heating it.

Do not post a sample unless the service has supplied and validated a postal collection kit. A clinic test and a post-vasectomy semen test also have different protocols, so use the correct instructions.

What are the current semen-analysis reference values?

NICE guideline NG257 uses reference values from the sixth edition of the World Health Organization laboratory manual.

Measure WHO reference value used by NICE What it describes
Semen volume1.4 ml or moreThe total fluid collected
pH7.2 or moreHow acidic or alkaline the semen is
Sperm concentration16 million per ml or moreThe number of sperm in each millilitre
Total sperm number39 million per ejaculate or moreThe estimated number in the whole sample
Total motility42% or moreThe proportion of sperm that move
Progressive motility30% or moreThe proportion moving forwards
Vitality54% or more live spermThe proportion of sperm that are alive
Normal morphology4% or moreThe proportion meeting strict normal-shape criteria

These figures are reference values, not targets and not diagnostic cut-offs. They describe the lower fifth centile in a reference population of men whose partners conceived within 12 months. Some men below a reference value conceive naturally, and some men above every reference value experience infertility.

How to understand common terms on the report

  • Oligozoospermia means a low sperm concentration.
  • Asthenozoospermia means reduced sperm motility.
  • Teratozoospermia means a reduced proportion of sperm meeting strict morphology criteria.
  • Azoospermia means no sperm were found in the ejaculate after the required laboratory examination.
  • Oligoasthenoteratozoospermia describes a combination of reduced concentration, movement and morphology.

These terms describe the laboratory findings. The cause and treatment options come from the wider fertility assessment.

Does sperm morphology of 4% mean 96% of sperm are defective?

No. Morphology is assessed against very strict laboratory criteria. A result of 4% means that four in every hundred sperm examined met every feature of the reference shape; it does not mean the others are genetically abnormal or incapable of fertilisation. Morphology should be interpreted with count, movement, the laboratory's quality standards and the wider fertility assessment.

Why can semen-analysis results vary?

Semen parameters naturally vary between ejaculates. The abstinence period, incomplete collection, transport delay, temperature, recent fever or illness and laboratory method may also affect a result. This is why an isolated abnormal test is normally confirmed before a diagnosis or major treatment decision.

Use the same laboratory for a repeat where possible, and follow its abstinence and collection instructions consistently.

When should a semen analysis be repeated?

If the first result is abnormal, NICE recommends a repeat confirmatory test. It should ideally be repeated after about three months, allowing the cycle of sperm formation to be completed. If the result shows azoospermia or severe oligozoospermia, the repeat should be performed as soon as possible rather than waiting three months.

A clinician may also repeat a test when collection was incomplete, transport conditions were outside the laboratory's requirements or the findings do not fit the clinical history.

What happens after an abnormal result?

The next step depends on which measures are affected and by how much. After two or more abnormal analyses, NICE recommends a physical examination of the scrotum and testes and consideration of testosterone and gonadotrophin blood tests.

Further tests are selected rather than automatic. They may include:

  • a testicular ultrasound when examination or history indicates it
  • urine testing when retrograde ejaculation or infection is suspected
  • genetic tests for defined patterns of azoospermia or very low sperm concentration
  • other blood tests guided by symptoms and the hormone results.

NICE advises against routine antisperm-antibody testing and sperm DNA-fragmentation testing in the standard UK fertility pathway.

Can a semen analysis be normal when conception is delayed?

Yes. Semen analysis measures selected features under laboratory conditions. It cannot assess every step required for natural conception, and fertility also depends on the reproductive health and age of the partner trying to become pregnant. Both partners should be assessed together.

Make sense of what comes next

Our guide to possible causes explains what an abnormal result can lead to. For questions about Heracles and your next step, contact the Heracles team. Keep the full laboratory report for the clinician reviewing your fertility.